Development of a Serological Test for Haemophilus ducreyi for Seroprevalence Studies
نویسندگان
چکیده
منابع مشابه
Development of a serological test for Haemophilus ducreyi for seroprevalence studies.
We developed a new enzyme immunoassay (rpEIA) for use in determining the seroprevalence of chancroid. Three highly conserved outer membrane proteins from Haemophilus ducreyi strain 35000 were cloned, overexpressed, and purified from Escherichia coli for use as antigens in the rpEIA. Serum specimens from patients with and without chancroid were assayed to determine optimum sensitivity and specif...
متن کاملDevelopment of a rapid immunodiagnostic test for Haemophilus ducreyi.
Haemophilus ducreyi is the etiologic agent of chancroid, a sexually transmitted disease that increases the rate of transmission of human immunodeficiency virus. Chancroid ulcerations are difficult to distinguish from those produced by syphilis and herpes. Diagnosis based solely on clinical grounds is inaccurate, and culture is insensitive. Highly sensitive PCR has largely superseded culture as ...
متن کاملNutritional studies of a virulent strain of Haemophilus ducreyi.
Haemophilm ducreyi has been described as a fastidious organism requiring blood for growth (Greenblatt, 1953). It has been successfully cultivated on clotted blood (Teague and Diebert, 1920) and on various artificial media enriched with blood, (Sanderson and Greenblatt, 1937; Beeson and Heyman, 1945; Beeson, 1946; Deacon et al., 1956). However, little is known concerning the specific nutritional...
متن کاملSheffield medium for cultivation of Haemophilus ducreyi.
Our interest in the role of Haemophilus ducreyi in genital ulceration led us to examine the various media commonly used for the cultivation of the organism. We describe an improved medium for the routine isolation of H ducreyi. In comparative studies using 50 test strains originally isolated in the United Kingdom, Canada, the United States of America, and Kenya, the new medium proved superior t...
متن کاملBiology of Haemophilus ducreyi.
The etiological agent of the sexually transmitted genital ulcer disease chancroid was first described in 1889 by Auguste Ducrey following repeated autoinoculation of purulent ulcer material from a series of patients. The organism was isolated on artificial media a decade later but has remained difficult to isolate consistently, resulting in controversy over its characteristics and role as the c...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2000
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.38.4.1520-1526.2000